cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota malang,
Jawa timur
INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 10 Documents
 1 
Search results for , issue "Vol. 1 No. 1 (2010)" : 10 Documents clear
Andrographolide Ameliorate Rheumatoid Arthritis by Promoting the Development of Regulatory T Cells Muhaimin Rifai'i
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Andrographolide is important material present in Andrographis paniculata. This material can promote T cell to develop into regulatory T cell, CD4+CD25+. CD4+CD25+ regulatory T (Treg) cells, a component of the innate immune response, which play a key role in the maintenance of self-tolerance, have become the focus of numerous studies over the last decade. These cells have the potential to be exploited to treat autoimmune disease. In this experiment we showed that the induction of regulatory cells in vivo has a healing effect on mice model of rheumatoid arthritis (RA). RA mice which is intraperitoneally injected with Andrographis paniculata shows the effect of recovery. CD4+CD25+ regulatory T cells actively control the properties of other immune cells by suppressing their functional activity to prevent autoimmunity. In this experiment we showed that active compound from Andrographis paniculata namely andrographolide can induce active regulatory T cell that has an efficacy to cure rheumatoid arthritis in mice model. Keywords: Andrographolide; Andrographis sp; Regulatory T cells; Rheumatoid arthritis
Significance of the Easy-to-use Water Quality Checker for Participative Environmental Monitoring and Experience Based Learning Akira Kikuchi; Luchman Hakim; Arien Heryansyah; Romaidi Romaidi
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Pack Test is a series of products of Kyoritsu Chemical-Check Laboratory Cooperation, Japan. It is easy-to-use, anybody can use at anywhere, low cost, nontoxic, safe, and professional-use, onetime use ion-selective color metric water quality checker. The aim of this research is to assess, then next, discuss the significance of applications of this tool. In this order, NH4-Pack Test was selected as an example for the discussions; although there are more than 60 parameters can be detected by pack test such as, COD, Cl-, NO3-, phosphate, hardness, pH, heavy metals, etc.. As for field survey, Ion chromatography was used to measure ammonium concentration of river water in Jakarta. Then detection range of the NH4-Pack Test was compared to the data, and Indonesian and Malaysian national water quality standard. River water quality of Jakarta was weekly degraded at upstream area, but it was seriously degraded at downstream area (up to 5-10 NH4-N mg/L). As for ammonium concentration, obviously the detection range and step of Pack Test was sufficient to assess the ammonium concentrations of rive water in Jakarta. Of course Pack Test is very simplified tool, environmental water quality standard of ammonia for class I in Indonesia and Class I and II in Malaysia were difficult to evaluate. However, it was obviously applicable to check treated effluent and Class III to V water quality of Malaysian environmental standard. Consequently, it is suggested to adopt a double standard policy of water quality monitoring, such as combination of "easy-to-use simplified" and "conventional-accurate". Because of low cost, and professional-convenient design, implementation of Pack Test will significant to empower on-site water quality monitoring in developing country, participative environmental awareness public programs, experience base environmental learning in schools, and other grass-rooted environmental activities.Keywords: Environmental awareness and education, Pack test, Simplified method, Southeastern Asian county
Segmental Duplications: A Possible Mechanism of Hominid Uplift through MicroRNA Diversification Maria A. Endriga; Aldrich Ivan Lois D. Burog; Denise Lauren V. Dalmacion; Custer C. Deocaris
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

MicroRNAs (miRNA) are ~21 nucleotide-long gene silencers. Segmental duplications (SD) are among the driving forces in acquiring new genes. Both miRNA and SD are believed to have played a significant role in evolution, particularly in the divergence of humans (Homo sapiens) from the chimpanzee (Pan troglodytes). This study determines the distribution of miRNAs in humans and in chimpanzees, and presents a hypothesis on its significance in the occurrence of segmental duplications. MiRNA sequences from miRBASE were subjected to BLAT and BLAST to determine if miRNAs are located in SD regions or not. Homology between miRNAs was determined with ClustalW. BLAST was then used to determine whether the non-homologous human miRNA are homologous to any other part of the chimpanzee genome. We found that all 695 human miRNAs are found exclusively in SD regions, and that 67 are de novo miRNAs. Thirteen are homologues of chimpanzee miRNA, and 11 were possibly derived from non-miRNA regions in chimp. Of these, 6 were located in SD regions of the chimpanzee genome. Results indicate that miRNA evolution occurs within regions of segmental duplication and suggest that the presence of miRNA duplicates allows more exposure to mutations that could necessitate diversification, and possibly evolution, through sub- and neofunctionalization.Keywords:
HOX Gene Promoter Prediction and Inter-genomic Comparison: An Evo-Devo Study Maria A. Endriga; Victoria Karenina R. de la Paz; Jezreel Marie G. Sazon; Elisa L. Co; Custer C. Deocaris
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Homeobox genes direct the anterior-posterior axis of the body plan in eukaryotic organisms. Promoter regions upstream of the Hox genes jumpstart the transcription process. CpG islands found within the promoter regions can cause silencing of these promoters. The locations of the promoter regions and the CpG islands of Homeo sapiens sapiens (human), Pan troglodytes (chimpanzee), Mus musculus (mouse), and Rattus norvegicus (brown rat) are compared and related to the possible influence on the specification of the mammalian body plan. The sequence of each gene in Hox clusters A-D of the mammals considered were retrieved from Ensembl and locations of promoter regions and CpG islands predicted using Exon Finder. The predicted promoter sequences were confirmed via BLAST and verified against the Eukaryotic Promoter Database. The significance of the locations was determined using the Kruskal-Wallis test. Among the four clusters, only promoter locations in cluster B showed significant difference. HOX B genes have been linked with the control of genes that direct the development of axial morphology, particularly of the vertebral column bones. The magnitude of variation among the body plans of closely-related species can thus be partially attributed to the promoter kind, location and number, and gene inactivation via CpG methylation.
The Control of Human Immunosystem by Using Paeony Root Drug Hideo Tsuboi; Muhaimin Rifa’i; Khaled Hossain; Izumi Nakashima; Haruhiko Suzuki
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Paeoniflorin (PF), isolated from paeony root, has been used as a herbal medicine for more than 1200 years in China, Korea and Japan for its anti-allergic, anti-inflamatory and immunoregulatory effects. In this study, we found that PF induces apoptosis in both murine T-lineage cells and human T-cell leukemia Jurkat cells. This apoptosis was mediated through the reduction of mitochondrial membrane potential, activation of caspase and fragmentation of DNA. Interestingly, PF induced generation of reactive oxygen species (ROS) and a reducing agent, dithiothreitol (DTT), and a ROS scavenger, N-acetyl cysteine (NAC), successfully attenuated the PF-induced apoptosis. Additionally, PF induced the phosphorylation of three mitogen-activated protein (MAP) family kinases, extracellular signal-regulated kinase, c-Jun amino-terminal kinase (JNK) and p38 MAP kinase. Curcumin, an anti-oxidant and JNK inhibitor, inhibited PF-induced apoptosis, suggesting the possible involvement of curcumin-sensitive JNK or other redox-sensitive elements in PF-induced apoptosis. These results partially explain the action mechanism of PF-containing paeony root as a herbal medicine.Keywords: paeoniflorin; paeony root; apoptosis; reactive oxygen species (ROS); redox; CD8+ CD122+ regulatory T cells
Potential Antibacterials Compounds of Lactic Acid Bacteria (LAB) from Quail Intestine (Coturnix japonica) in Inhibition Growth of Escherichia coli and Salmonella typhimurium Pupimadita Tizar Afdora; Tri Ardiyati; Osfar Sjofjan; Umi Kalsum
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Quail (Coturnix japonica) is a bird that have high protein content, but vulnerable to digestive diseases. The purpose of this research was to determine the ability of antibacterial compounds of LAB from intestinal quail origin in bacterial growth inhibition test. This research used Completely Randomized Design (CRD) with variable concentrations of Cell Free Supernatant (CFS) for 10, 20, 30, 40, 50, and 60% and bacterial pathogens of the digestive tract of quail (S. typhimurium, E. coli of human origin, and E. coli of bird origin) by using the Minimum inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC). The data obtained were analyzed by Analysis of Variance (ANOVA). The results obtained showed that the bacterium Lactobacillus fermentum and L. salivarius derived from quail intestine can produce antibacterial compounds that could inhibit the growth of Salmonella typhimurium, Escherichia coli (human), and E. coli (bird). Minimum concentration of the addition of CFS from L. fermentum in inhibiting the growth of tested bacteria was 30% for S. typhimurium, 30% for E. coli (human), and 20% for E. coli (bird). While the addition of CFS minimum concentration of L. salivarius in inhibiting the growth of tested bacteria was 20% for S. typhimurium, 20% for E. coli (human), and 10% for E. coli (bird). Keywords: Antibacterials, Lactic Acid Bacteria (LAB), Escherichia coli, Quail, and Salmonella typhimurium.
Development of Green Resin Using Solid Waste Protein Soybean Curd “Tofu” Production Sujito Sujito
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

One of the most concerns associated with many commercially available composites is that they used of non-degradable resins and fibers that primarily made using non-degradable, petroleum-based chemicals as feed stock. These conditions will create a serious problem in term of waste disposal after end of their life. Unlike petroleum, plant based protein and starches are yearly renewable. These resins are increasingly developed for various applications as replacements for non-degradable petroleum based resins. In addition, these resins may be easily composted after their life. In this study, Soybean Pulp Hemi-cellulose (SPH) was modified by cross-linking it with glutaraldehyde (GA). The modified SPH resins were characterized for its surface morphology, tensile and mass losses or biodegradability properties. The effect of GA on the surface morphology, tensile and biodegradability of the SPH resins were discussed. The SPH resins showed improved surface morphology and ductility. However, the increasing the GA content reduces the Young's modulus of the SPH resins. The SPH resins exhibited fracture stress point and Young's modulus maximum of and 3.02 MN/m2, respectively, and biodegradability of 40.42% after 30 days placed on the open air. These properties seem to be sufficient for developing green composites from the SPH resins reinforced with natural fiber for indoor structural applications. Keywords: Green Resin, Soybean Pulp Hemi-cellulose (SPH), Mechanical Properties, Biodegradation.
Blooming Stimulation of Microcystis in Sutami Reservoir Using Nutrients Nitrate and Phosphate in Different ratio Catur Retnaningdyah; Suharjono Suharjono; Agoes Soegianto; Bambang Irawan
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Microalgae Cyanobacteria from the genus Microcystis has the potential to bloom in Sutami reservoirs that located in Malang, Indonesia at a particular time. Microcystis is a notorious species because it can produce toxins that are dangerous to other organisms. The objective of this research is to determine the influence of different ratios of Nitrate and Phosphate on the growth of Microcystis in Sutami reservoir. The study was conducted from April to November 2009 and was carried out in situ in the reservoir Sutami with quasi experiment using a completely randomized design. Sutami reservoir water which already contained plankton community therein, including Microcystis were treated with five variations ratio of Nitrate and Phosphate (10, 20, 40, 80, and 160) and untreated water as control. Experiments carried out by using a transparent plastic bag diameter 1 m and 1.5 m long, inserted into the water and be immersed in the water by using bamboo. Calculation of Microcystis abundance and physic-chemical quality of water is done every three days for a month. The results showed that the higher concentration of orthophosphate in the media water of Sutami reservoir have the maximum abundance of Microcystis. Abundance of Microcystis grown in Sutami reservoirs have positively correlated with actual levels of nitrate, nitrite, total phosphate, conductivity, pH, temperature, ratio of Nitrate to Phosphate treatment and water KMnO4 value.Keywords: Blooming Microcystis, the ratio of nutrients Nitrate to Phosphate, Sutami reservoir
Eusideroxylon zwageri (Ulin) as Key Species in Two Zones of Sangkima Rain Forest, Kutai National Park, East Kalimantan Jehan Ramdani Haryati; Intan N. Azizah; Endang Arisoesilaningsih
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Aim of this research was to study trees' diversity quality in primary and intensively used forest of Sangkima area in Kutai National Park (TNK), East Kalimantan (Borneo). Field observation was carried out by vegetation analysis using 17 sampling plots of 25x25 m2. Community structure of each forest was determined by calculating trees' density, basal area, frequency, important value and stratification of species. While trees diversity was estimated by taxa richness, Shannon-Wiener diversity index, and rate of endemism. Both forests were compared by Morisita community similarity index. Data were tabulated by Microsoft Excel 2007 and statistically analyzed by PCA method and supported by hierarchical cluster analysis in SPSS 15.00 for windows. The result showed that diversity quality in primary and intensively used forest of Sangkima TNK was high, indicated by similar stratification. The forests were composed by A stratum trees of > 30 m high to ground cover plants, but they were dominated by B stratum trees of 20-30m high. Primary forest's formation was Eusideroxylon zwageri-Mixed Dipterocarpaceae, while intensively used forest's formation was E. zwageri. Taxa richness of both forests was not different significantly. In the primary forest was found 34 species, 25 families and 16 orders, while intensively used forest was found 36 species, 20 families and 13 orders. Diversity Index of primary forest (H=4.57) was slightly higher than secondary forest (H=4.28). Rate of endemism of both forests reached 100%. Eusideroxylon zwageri and Cananga odorata were co-dominant in the Borneo rain forest. Tree of E. zwageri showed a biggest trunk and largest canopy. Cananga odorata showed a high density in both zones. Luxurious jungle performed A to E strata, but B stratum was dominant. Based on those five characters, trees' diversity quality in Sangkima was still high. Bi plot analysis showed that trees' community structure of both forests composed by three groups. In secondary forest, E. zwageri was a key species and characterized by high important value, while species C. odorata, Dyospiros borneensis and Dillenia beccariana found in a high distribution, otherwise other trees were classified into the 3rd group. Comparing to the secondary forest, even though E. zwageri was also as dominant tree, but the virgin one showed more species growing in a better density, distribution and dominance.Keywords: Tree, Borneo, rain forest, community structure, quality diversity, Kutai National Park
Assessment of bio-activities of the crude extract and components of Withania somnifera leaves by bioinformatics Nashi Widodo; Didik Priyandoko; Custer C. Deocaris; Renu Wadhwa; Sunil C. Kaul
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Traditional herbal medicines are now increasingly being appreciated with Western models of integrative health sciences and evidence-based approach both in the basic research and clinic scenario. Ashwagandha is a commonly used plant in Ayurvedic, Indian traditional medicine. Medicinal value of Ashwagandha (WithaniasomniferaDunal) extends from anti-inflammatory, anti-arthritic, anti-rheumatic, rejuvenation and anti-cancer. Based on the belief that holistic multi-site mechanism of action offers greater chance of success, the traditional Ayurvedicmedicine practices the use of whole herb or its crude extract. It opposes with the mainstream of pharmaceutical industry that uses single and purified molecules. In the present study, we used bioinformatics approach to reveal the mechanism of action of (i) crude extract of Ashwagandha leaf extract and its purified components, (ii) Withanone and (iii) Withaferin A. Whereas p53-p21 was identified as a common signaling pathway for the three kinds of reagents, specific signaling pathways for Withaferin-A and Withanone were identified. Whereas the crude extract and Withanone were selectively toxic to human cancer cells, Withaferin A showed cytotoxicity to the normal cells too. The study suggested that the crude extract or a combinational formulamay be a superior and safenatural reagent for cancer treatment.

Page 1 of 1 | Total Record : 10

 1 

Filter by Year

2010 2010


Reset
Filter By Issues
All Issue Vol. 13 No. 3 (2023) Vol. 13 No. 2 (2023) Vol. 13 No. 1 (2023) Vol. 12 No. 3 (2022) Vol. 12 No. 2 (2022) Vol. 12 No. 1 (2022) Vol 11, No 3 (2021) Vol. 11 No. 3 (2021) Vol. 11 No. 2 (2021) Vol 11, No 2 (2021) Vol. 11 No. 1 (2021) Vol 11, No 1 (2021) Vol 10, No 3 (2020) Vol. 10 No. 3 (2020) Vol 10, No 2 (2020) Vol. 10 No. 2 (2020) Vol. 10 No. 1 (2020) Vol 10, No 1 (2020) Vol 9, No 3 (2019) Vol. 9 No. 3 (2019) Vol. 9 No. 2 (2019) Vol 9, No 2 (2019) Vol. 9 No. 1 (2019) Vol 9, No 1 (2019) Vol 8, No 3 (2018) Vol. 8 No. 3 (2018) Vol 8, No 2 (2018) Vol. 8 No. 2 (2018) Vol. 8 No. 1 (2018) Vol 8, No 1 (2018) Vol 7, No 3 (2017) Vol. 7 No. 3 (2017) Vol. 7 No. 2 (2017) Vol 7, No 2 (2017) Vol 7, No 1 (2017) Vol. 7 No. 1 (2017) Vol 6, No 3 (2016) Vol. 6 No. 3 (2016) Vol 6, No 2 (2016) Vol. 6 No. 2 (2016) Vol 6, No 1 (2016) Vol. 6 No. 1 (2016) Vol. 5 No. 3 (2015) Vol 5, No 3 (2015) Vol 5, No 2 (2015) Vol. 5 No. 2 (2015) Vol 5, No 1 (2015) Vol. 5 No. 1 (2015) Vol. 4 No. 3 (2014) Vol 4, No 3 (2014) Vol. 4 No. 2 (2014) Vol 4, No 2 (2014) Vol. 4 No. 1 (2014) Vol 4, No 1 (2014) Vol. 3 No. 3 (2013) Vol 3, No 3 (2013) Vol 3, No 2 (2013) Vol. 3 No. 2 (2013) Vol 3, No 1 (2013) Vol. 3 No. 1 (2013) Vol. 2 No. 3 (2012) Vol 2, No 3 (2012) Vol. 2 No. 2 (2012) Vol 2, No 2 (2012) Vol. 2 No. 1 (2012) Vol 2, No 1 (2012) Vol 1, No 2 (2011) Vol. 1 No. 2 (2011) Vol 1, No 1 (2010) Vol. 1 No. 1 (2010) More Issue